The Effect of the Oestrous Cycle and of Oestradiol on the Breakdown of Seminal Glycerylphosphorylcholine by Secretions of the Rat Uterus.

Glycerylphosphorylcholine (GPO) diesterase activity and protein concentration in the rinsings of the rat uterus were greater during pro-oestrus and oestrus than at metoestrus or dioestrus. Oestradiol-3, 17~ significantly stimulated the secretion of this enzyme in ovariectomized rats and maximum levels of activity were observed 24-36 hr after a single injection of the oestrogen. The protein content of the uterine rinsings was also increased by oestradiol, but was greatest 12 hr after injection. The level of GPO diesterase activity was independent of changes in the weight or vascularity of the uterus.